The Restriction endonucleases recognizes a specific base sequence of four to eight bases in double-stranded DNA and cleaves both strands of the duplex.
There are known type of restriction endonucleases : type I,II and III. Type II enzymes are frequently used in the rDNA technology. Type I and type III are not use because these type enzymes cleaves the DNA far from the recognition sites.
The Restriction endonuclease recognizes particular specific sequences are 4 to 6 nucleotides recognize by the type II enzymes because these enzymes only cleave at this site. The palindrome sequence is “Two-fold symmetry” as shown above video.
Restriction Endonuclease
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