Principles of Gel Electrophoresis

Electrophoresis: How to Read Results

Electrophoretic separation of Proteins

Electrophoretic separation of Proteins

DNA Polymerase

DNA Polymerase enzyme mechanism

DNA Polymerase enzyme

Restriction Endonucleases

The Restriction endonucleases recognizes a specific base sequence of four to eight bases in double-stranded DNA and cleaves both strands of the duplex.

There are known type of restriction endonucleases : type I,II and III. Type II enzymes are frequently used in the rDNA technology. Type I and type III are not use because these type enzymes cleaves the DNA far from the recognition sites.

The Restriction endonuclease recognizes particular specific sequences are 4 to 6 nucleotides recognize by the type II enzymes because these enzymes only cleave at this site. The palindrome sequence is “Two-fold symmetry” as shown above video.

Restriction Endonuclease