Wednesday, January 30, 2013
How to Make an SDS-PAGE gel
Alright so here's a quick video on how to cast an SDS-PAGE gel. Although recipes can vary, the ingredients shown here are almost always used. Remember: always add TEMED last, and pour into plates immediately after!
Already know how to make a gel, but have problems with leaky gels? Check out our other video on How to Avoid a Leaky SDS-PAGE gel: See the video here
Tuesday, December 14, 2010
Restriction Endonucleases
Labels: Animated Tutorials, Molecular Biology, Recombinant DNA Technology, Student Notes, Video lectures
The Restriction endonucleases recognizes a specific base sequence of four to eight bases in double-stranded DNA and cleaves both strands of the duplex.
There are known type of restriction endonucleases : type I,II and III. Type II enzymes are frequently used in the rDNA technology. Type I and type III are not use because these type enzymes cleaves the DNA far from the recognition sites.
The Restriction endonuclease recognizes particular specific sequences are 4 to 6 nucleotides recognize by the type II enzymes because these enzymes only cleave at this site. The palindrome sequence is “Two-fold symmetry” as shown above video.
